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Important Topic of Plant Tissue Culture Biology Class 12 How is Plant tissue culture done? The first step is excising/ removing a small part of plant tissue, like the leaves, root or shoot of a plant then placing the cut-out part or explant in a suitable nutrient medium followed by allowing the explants to multiply and form a group of tissues(undifferentiated tissue) called  callus . The callus is then allowed to multiply to form many plantlets which are then transferred to a different container for rapid multiplication. Finally, the developed plantlets are transplanted to the soil for normal growth. Therefore, tissue culture is performed using a small part of a plant to develop many plants and so is also called  micropropagation . As all these plants are developed from the same part of the parent plant, they are genetically identical and called  somaclones . Micropropagation, or tissue culture, is employed in the large-scale production of tomato, banana, and apple. Plant ti